human colorectal cancer cell lines  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Network Pharmacology and Molecular Docking-Based Approach Revealing the Potential Anticancer Compounds and Molecular Mechanisms of Paris polyphylla Against Colorectal Cancer

doi: 10.3390/ijms27093874

Figure Lengend Snippet: Cytotoxic effects of Paris polyphylla rhizome extract (PPRE) on colorectal cancer cells. SW480 ( A ) and HCT116 ( B ) cells were treated with increasing concentrations of PPRE (0–80 µg/mL) for 24 and 48 h. Data are presented as mean ± SD from three independent experiments. Statistical significance compared with the untreated control group was determined using one-way ANOVA followed by Tukey’s multiple comparison test (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Human colorectal cancer cell lines SW480 and HCT116 (ATCC, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Control, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Network Pharmacology and Molecular Docking-Based Approach Revealing the Potential Anticancer Compounds and Molecular Mechanisms of Paris polyphylla Against Colorectal Cancer

doi: 10.3390/ijms27093874

Figure Lengend Snippet: qRT-PCR Validation of STAT3, EGFR, SRC, IL-6, and AKT1 mRNA expression in SW480 ( A – E ) and HCT116 ( F – J ) cells following treatment with PPRE (0–10 µg/mL). Gene expression levels were normalized to the internal control and expressed as fold change relative to untreated control (0 µg/mL). Data are presented as mean ± SD of three independent experiments. Statistical significance versus the untreated control was determined using one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Human colorectal cancer cell lines SW480 and HCT116 (ATCC, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Gene Expression, Control

Journal: RSC Advances

Article Title: Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities

doi: 10.1039/d6ra01587e

Figure Lengend Snippet: Cytotoxic and anti-cancer activity of the N1: (A) dose-dependent inhibition of cell proliferation in murine colon carcinoma cell lines (CT26 and MC-38) and human colorectal cancer cell line (HCT-15) upon treatment with increasing concentrations of N1, determined by MTT assay; (B) cell viability of normal murine fibroblast cells (NIH-3T3) following treatment with N1 at indicated concentrations, demonstrating minimal cytotoxicity; (C–E) dose–response curves showing percentage growth inhibition and corresponding IC 50 values for CT26 (C), MC-38 (D), and HCT-15 (E) cells. IC 50 values are calculated by nonlinear regression analysis using GraphPad Prism. Data are expressed as mean ± SD ( n = 3).

Article Snippet: The murine colon carcinoma cell lines CT26, human colorectal cancer cell line HCT-15, and normal murine fibroblast cell line NIH-3T3 were procured from ATCC (USA).

Techniques: Activity Assay, Inhibition, MTT Assay

Journal: RSC Advances

Article Title: Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities

doi: 10.1039/d6ra01587e

Figure Lengend Snippet: Effect of ROS scavenging and phosphatase inhibition on N1-induced cytotoxicity: (A) percentage cytotoxicity in CT26 cells treated with N1 (0.04 and 0.08 µg mL −1 ) in the presence or absence of N -acetyl cysteine (NAC, 5 mM) and PhosSTOP phosphatase inhibitor (1×); (B) corresponding cytotoxicity in HCT-15 cells under similar treatment conditions. NAC pre-treatment significantly reduces N1-induced cytotoxicity, indicating the involvement of ROS, while PhosSTOP treatment partially attenuates cytotoxicity, suggesting a possible contribution of phosphate-related processes. Data are expressed as mean ± SD ( n = 3; *** p < 0.001, **** p < 0.0001 vs. N1-treated group).

Article Snippet: The murine colon carcinoma cell lines CT26, human colorectal cancer cell line HCT-15, and normal murine fibroblast cell line NIH-3T3 were procured from ATCC (USA).

Techniques: Inhibition